Vector Cloning
Vector Cloning
As the world's largest provider of custom vector cloning services, VectorBuilder can practically clone any custom DNA vector tailored to your research needs. About 200 vector systems on our platform have been optimized through extensive R&D work, and all have been validated by in vitro and where applicable, in vivo testing. With the unbeatable price and rapid turnaround, outsourcing cloning to VectorBuilder is often cheaper than DIY cloning in the lab in terms of reagent and labor cost, let alone saving your time, effort and the frustration associated with cloning.
Besides molecular cloning of DNA vectors, we also offer many related services such as plasmid DNA preparation, virus packaging (lentivirus, AAV, adenovirus, MMLV, baculovirus, etc.), library construction, BAC recombineering, mutagenesis, stable cell line generation and RNA preparation.
Deliverables
The default deliverable format is E. coli glycerol stock. Upon request, small amounts of plasmid DNA can be provided if available without additional charge. Multiple scales of high-quality plasmid DNA can be purchased additionally: Miniprep (> 10 ug), Midiprep (>100 ug), Maxiprep (> 300 ug), Megaprep (>1 mg) and Gigaprep (>10 mg). For viral vectors, virus packaging offered at various scales is available as a downstream service.
Quality control
All vectors cloned by VectorBuilder come with a 100% sequence guarantee. Our vectors undergo stringent quality control to ensure that they contain the correct sequence exactly as designed. Common QC includes DNA quantification, A260/280 measurement, electrophoresis of undigested and restriction enzyme digested plasmid DNA, Sanger sequencing, recovering bacteria from glycerol stocks, and re-transformation.
Customers-supplied materials
If customer-supplied materials such as backbones or gene templates are needed, please submit your materials information on "Support" > "Materials Submission Form". Please strictly follow our guidelines to set up shipment to avoid any delay or damage of the materials. All customer-supplied materials undergo mandatory QC by VectorBuilder which may incur an additional QC charge starting from $120 per item, depending on the type and usage of item. Please note that production cannot be initiated until customer-supplied materials pass QC.
How to Order
